Review



fitc avidin  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Thermo Fisher fitc avidin
    Fitc Avidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Paraformaldehyde%2C+90%25%2C+pure/pmc13181568-56-4-11
    Average 94 stars, based on 1 article reviews
    fitc avidin - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation
    Article Snippet: .. At 48 h post-transfection, cells were treated with TNFα (10 ng/mL) or PBS for 3 h. FITC-avidin (Cat# A821, Molecular Probes, concentration 25 μg/mL) was then added and incubated for 210 s. Cultures were rinsed, fixed with 4% paraformaldehyde in HBSS and imaged (6 images per condition) using an Olympus epifluorescence inverted microscope with a 10× objective by a blinded investigator. ..

    Incubation:

    Article Title: Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation
    Article Snippet: .. At 48 h post-transfection, cells were treated with TNFα (10 ng/mL) or PBS for 3 h. FITC-avidin (Cat# A821, Molecular Probes, concentration 25 μg/mL) was then added and incubated for 210 s. Cultures were rinsed, fixed with 4% paraformaldehyde in HBSS and imaged (6 images per condition) using an Olympus epifluorescence inverted microscope with a 10× objective by a blinded investigator. ..

    Article Title: Choroid plexus mast cells drive tumor-associated hydrocephalus.
    Article Snippet: Article Choroid plexus mast cells drive tumor-associated hydrocephalus

    Inverted Microscopy:

    Article Title: Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation
    Article Snippet: .. At 48 h post-transfection, cells were treated with TNFα (10 ng/mL) or PBS for 3 h. FITC-avidin (Cat# A821, Molecular Probes, concentration 25 μg/mL) was then added and incubated for 210 s. Cultures were rinsed, fixed with 4% paraformaldehyde in HBSS and imaged (6 images per condition) using an Olympus epifluorescence inverted microscope with a 10× objective by a blinded investigator. ..

    Saline:

    Article Title: Mast cell-derived prostaglandin D 2 limits the subcutaneous absorption of honey bee venom in mice
    Article Snippet: .. 11 12 Reagents 13 The following reagents were obtained from the indicated suppliers: Isoflurane, 14 ethanol, paraformaldehyde, n-hexane, methanol, formic acid, ethyl acetate, acetic acid, 15 acetonitrile, HEPES (Wako), Roswell Park Memorial Institute medium (RPMI), 16 penicillin-streptomycin, fetal bovine serum (Nichirei Biosciences Inc.), interleukin-3 (IL-17 3) (Wako, prospec), Bee Venom from Apis mellifera (Sigma and Latoxan), 2-18 mercaptoethanol, p-nitrophenyl N acetyl--D-glucosamide, peanuts oil, tamoxifen, Cy3 19 conjugated anti mouse -SMA (Sigma), Otsuka Normal Saline (Otsuka Pharmaceutical 20 Factory), Triton X-100 (MP biomedicals), Prostaglandin D2-d4 (Cayman chemical), rat 21 anti-PECAM-1 (BD biosciences), rabbit anti-Lyve1 (Abcam), goat anti-VE cadherin 22 (SANTA CRUZ), FITC-Avidin, goat anti-rabbit Alexa Fluor 546, goat anti-rat Alexa 23 Fluor 647, donkey anti-goat Alexa Fluor 488 (life technologies), mounting medium for 24 immunofluorescent microscopy (KPL), mounting medium for fluorescence with DAPI 25 (VECTOR), TRITC-dextran 2000 (TdB Consultancy), FITC-5 (TCI), DCTM Protein 26 Assay (Bio-Rad), bovine serum albumin (prospec), normal goat serum, normal donkey 27 serum (Chemicon) and melittin (GenScript). .. 28 In the preliminary experiments to determine the high dose (sublethal) of each 29 BV, we found that the lethal dose of BV (Sigma-Aldrich, V3375, lot SLBH1334V) was 30 1200 ug, while the that of BV (Latoxan, L4105B) was 680 ug.

    Microscopy:

    Article Title: Mast cell-derived prostaglandin D 2 limits the subcutaneous absorption of honey bee venom in mice
    Article Snippet: .. 11 12 Reagents 13 The following reagents were obtained from the indicated suppliers: Isoflurane, 14 ethanol, paraformaldehyde, n-hexane, methanol, formic acid, ethyl acetate, acetic acid, 15 acetonitrile, HEPES (Wako), Roswell Park Memorial Institute medium (RPMI), 16 penicillin-streptomycin, fetal bovine serum (Nichirei Biosciences Inc.), interleukin-3 (IL-17 3) (Wako, prospec), Bee Venom from Apis mellifera (Sigma and Latoxan), 2-18 mercaptoethanol, p-nitrophenyl N acetyl--D-glucosamide, peanuts oil, tamoxifen, Cy3 19 conjugated anti mouse -SMA (Sigma), Otsuka Normal Saline (Otsuka Pharmaceutical 20 Factory), Triton X-100 (MP biomedicals), Prostaglandin D2-d4 (Cayman chemical), rat 21 anti-PECAM-1 (BD biosciences), rabbit anti-Lyve1 (Abcam), goat anti-VE cadherin 22 (SANTA CRUZ), FITC-Avidin, goat anti-rabbit Alexa Fluor 546, goat anti-rat Alexa 23 Fluor 647, donkey anti-goat Alexa Fluor 488 (life technologies), mounting medium for 24 immunofluorescent microscopy (KPL), mounting medium for fluorescence with DAPI 25 (VECTOR), TRITC-dextran 2000 (TdB Consultancy), FITC-5 (TCI), DCTM Protein 26 Assay (Bio-Rad), bovine serum albumin (prospec), normal goat serum, normal donkey 27 serum (Chemicon) and melittin (GenScript). .. 28 In the preliminary experiments to determine the high dose (sublethal) of each 29 BV, we found that the lethal dose of BV (Sigma-Aldrich, V3375, lot SLBH1334V) was 30 1200 ug, while the that of BV (Latoxan, L4105B) was 680 ug.

    Fluorescence:

    Article Title: Mast cell-derived prostaglandin D 2 limits the subcutaneous absorption of honey bee venom in mice
    Article Snippet: .. 11 12 Reagents 13 The following reagents were obtained from the indicated suppliers: Isoflurane, 14 ethanol, paraformaldehyde, n-hexane, methanol, formic acid, ethyl acetate, acetic acid, 15 acetonitrile, HEPES (Wako), Roswell Park Memorial Institute medium (RPMI), 16 penicillin-streptomycin, fetal bovine serum (Nichirei Biosciences Inc.), interleukin-3 (IL-17 3) (Wako, prospec), Bee Venom from Apis mellifera (Sigma and Latoxan), 2-18 mercaptoethanol, p-nitrophenyl N acetyl--D-glucosamide, peanuts oil, tamoxifen, Cy3 19 conjugated anti mouse -SMA (Sigma), Otsuka Normal Saline (Otsuka Pharmaceutical 20 Factory), Triton X-100 (MP biomedicals), Prostaglandin D2-d4 (Cayman chemical), rat 21 anti-PECAM-1 (BD biosciences), rabbit anti-Lyve1 (Abcam), goat anti-VE cadherin 22 (SANTA CRUZ), FITC-Avidin, goat anti-rabbit Alexa Fluor 546, goat anti-rat Alexa 23 Fluor 647, donkey anti-goat Alexa Fluor 488 (life technologies), mounting medium for 24 immunofluorescent microscopy (KPL), mounting medium for fluorescence with DAPI 25 (VECTOR), TRITC-dextran 2000 (TdB Consultancy), FITC-5 (TCI), DCTM Protein 26 Assay (Bio-Rad), bovine serum albumin (prospec), normal goat serum, normal donkey 27 serum (Chemicon) and melittin (GenScript). .. 28 In the preliminary experiments to determine the high dose (sublethal) of each 29 BV, we found that the lethal dose of BV (Sigma-Aldrich, V3375, lot SLBH1334V) was 30 1200 ug, while the that of BV (Latoxan, L4105B) was 680 ug.



    Similar Products

    fitc avidin  (Vector Laboratories)
    94
    Vector Laboratories fitc avidin
    Fitc Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/10__1042_slash_cs20261759-133-6-9
    Average 94 stars, based on 1 article reviews
    fitc avidin - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    fitc avidin  (Thermo Fisher)
    94
    Thermo Fisher fitc avidin
    Fitc Avidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Paraformaldehyde%2C+90%25%2C+pure/pmc13181568-56-4-11
    Average 94 stars, based on 1 article reviews
    fitc avidin - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    avidin fitc  (Thermo Fisher)
    96
    Thermo Fisher avidin fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Avidin/pmc12834844-154-22-23
    Average 96 stars, based on 1 article reviews
    avidin fitc - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    fluorescein isothiocyanate conjugated avidin fitc avidin  (Vector Laboratories)
    94
    Vector Laboratories fluorescein isothiocyanate conjugated avidin fitc avidin
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Fluorescein Isothiocyanate Conjugated Avidin Fitc Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pmc12736424-166-28-32
    Average 94 stars, based on 1 article reviews
    fluorescein isothiocyanate conjugated avidin fitc avidin - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    fitc conjugated avidin  (Thermo Fisher)
    99
    Thermo Fisher fitc conjugated avidin
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Fitc Conjugated Avidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Avidin/pm41378780-79-5-11
    Average 99 stars, based on 1 article reviews
    fitc conjugated avidin - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    avidin fitc  (Vector Laboratories)
    94
    Vector Laboratories avidin fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pm41375453-68-6-7
    Average 94 stars, based on 1 article reviews
    avidin fitc - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    avidin conjugated fluorescein isothiocyanate fitc  (Vector Laboratories)
    94
    Vector Laboratories avidin conjugated fluorescein isothiocyanate fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Conjugated Fluorescein Isothiocyanate Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pmc12738967-378-38-42
    Average 94 stars, based on 1 article reviews
    avidin conjugated fluorescein isothiocyanate fitc - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show avidin–FITC-positive degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.

    Journal: Biotechnology Reports

    Article Title: Production and characterization of rNGFSP: a recombinant fusion immunogen eliciting dual anti-NGF and anti-Substance P therapeutic antibodies for Degenerative Joint Disease

    doi: 10.1016/j.btre.2026.e00946

    Figure Lengend Snippet: Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show avidin–FITC-positive degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.

    Article Snippet: After stimulation, cells were centrifuged (1000 × g, 10 min, 4 °C), resuspended in 150 μL PBS, and stained with 2 μg/mL Avidin-FITC (Thermo Scientific, #A821) for 20 min at room temperature.

    Techniques: Functional Assay, Neutralization, Binding Assay, Inhibition, Enzyme-linked Immunosorbent Assay, Incubation, Positive Control, Flow Cytometry, Avidin-Biotin Assay, Confocal Microscopy, Staining